Effect of the extract from cortinarius rufo-olivaceus on antioxidation activities in H22 tumor bearing mouse
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摘要: 通过研究贺兰山紫蘑不同提取物对H22荷瘤小鼠体内抗氧化酶系统的影响,探讨其可能的抗肿瘤机制。给予紫蘑石油醚、乙酸乙酯、正丁醇提取物、紫蘑粗多糖、阳性对照香菇多糖灌胃及氟尿嘧啶腹腔注射后,采用比色法分别测定小鼠肝组织中超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的活性。结果表明,与阳性对照相比,紫蘑提取物均可不同程度提高荷瘤小鼠SOD、CAT、GSH-Px的活性,降低MDA含量,其中尤以乙酸乙酯部位和紫蘑粗多糖最为显著,说明贺兰山紫蘑可通过提高荷瘤小鼠抗氧化酶系统的活力,发挥其抗肿瘤机制。Abstract: To study the antioxidation activities of the different extract from cortinarius rufo-olivaceus via H22-bearing mice, discussed the possible mechanism of anti-tumor.The colorimetric analysis was adopted to determine the level of superoxide dismutase (SOD) , malondialdehyde (MDA) , calalase (CAT) and glutathione peroxidase (GSH-Px) in liver of H22-bearing mice after by giving petroleum ether, ethyl acetate, butanol phase extractions.Different extractions from corinairus rufo-olivaceus could increase the activities of SOD, MDA, CAT, GSH-Px and decrease the level of MDA especially the ethyl acetate part and polysaccharide.The result showed that the extract of corinairus rufo-olivaceus could obviously enhance the antioxidant acticity and prevent lipid peroxidation damage in H22-bearing mouse.
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