紫荆花红色素对模型动物血糖和血脂的影响研究
详细信息Study of red pigment from flowers of Cercis chinensis Bge. on blood glucose and serum lipid levels in model rats
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摘要: 目的:观察紫荆花红色素对模型动物血糖和血脂水平的影响。方法:腹腔注射四氧嘧啶制备糖尿病小鼠模型,灌胃高脂乳剂制备高脂血症大鼠模型。将动物分成对照和高、中、低不同剂量组,按动物体重给药实验。强生血糖仪测定小鼠血糖浓度,试剂盒法测定血清TC、TG、HDL-C和LDL-C等生化指标。结果:四氧嘧啶致糖尿病小鼠造模成功,紫荆花红色素各剂量组使小鼠体重显著增大,高、中剂量组使血糖显著降低（p<0.01）。高脂乳剂致SD大鼠高脂血症造模成功,红色素各剂量组均使大鼠血清TC和LDL-C水平显著降低,中、高组显著降低TG水平和LDL-C/HDL-C比值（p<0.05）,高剂量组对HDL-C水平具有明显的调节作用（p<0.01）。结论:紫荆花红色素对四氧嘧啶致糖尿病小鼠血糖水平和高脂血症大鼠的血脂水平有显著调节作用。Abstract: Objective: To explore the effect of red pigment from flowers of Cercis chinensis Bge. on blood glucose and serum lipid levels in model rats. Method: The diabetes mellitus rat model was induced with alloxan ( ALX) by abdominal cavity injecting and hyperlipidemia by intragastric administration of high-fat cream in SD rats.Animals were divided into control group and different doses groups ( high, middle and low) , and dosed according to body weight of rats. The serum glucose consistence was determined by blood glucose monitor and serum TC, TG, HDL-C and LDL-C were detected by corresponding reagent box.Results: Diabetic model rats were treated with alloxan successfully.Each dose group of red pigment from flowers of Cercis chinensis Bge. could increase body weight and middle dose, high dose all can decrease the blood glucose of diabetic rats markedly (p < 0.01) . The hyperlipoidemia model rats were treated with high-fat cream successfully. The red pigment could decrease the serum contents of TC and LDL-C significantly (p < 0.05) , middle dose and high dose could decrease the levels of TG (p < 0.05) and the value of LDL-C/HDL-C and arteriosclerotic index, high dose increase serum level of HDL-C obviously (p < 0.01) . Conclusion: The red pigment from flowers of Cercis chinensis Bge. could regulate blood glucose of diabetic rats treated with alloxan and the levels of hyperlipidemia rat treated with high-fat cream obviously.
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[1] 王金亭, 吴广庆, 鞠秀萍.紫荆花红色素化学结构的初步鉴定[J].中国食品添加剂, 2010 (4) :97-100. [2] 王金亭, 吴广庆, 鞠秀萍, 等.紫荆花红色素稳定性的研究[J].西北农业学报, 2009, 18 (5) :280-283. [3] 吴广庆, 王金亭, 鞠秀萍.紫荆花红色素体外抗氧化活性的研究[J].江苏农业科学, 2009 (4) :317-318, 340. [4] 王金亭.紫荆花红色素抑菌和体内抗氧化活性的研究[J].食品科技, 2011, 36 (9) :238-241. [5] Weiguang Y, Casimir C A, Joan Fischer, et al.Effects of phenolic compounds in blueberries and muscadine grapes on HepG2cell viability and apoptosis[J].Food Research International, 2006, 39 (5) :628-638.
[6] 李颖畅, 孟宪军, 孙靖靖, 等.蓝莓花色苷的降血脂和抗氧化作用[J].食品与发酵工业, 2008 (10) :46-47. [7] 刘荣, 赵静, 王振宇, 等.笃斯越桔花色苷对高脂血症大鼠血脂水平的影响[J].食品工业科技, 2011, 32 (5) :381-383. [8] Zhenyu W.Impact of anthocyanin from Malva sylvestris on plasma lipids and free radical[J].Journal of Forestry Research, 2005, 16 (3) :228-232.
[9] 孙晓侠.紫甘薯花色苷结构鉴定及抗氧化、降血糖功能的研究[D].天津:天津科技大学, 2006. [10] 张继曼.黑豆红花色苷的优化提取及其降血糖作用的研究[D].合肥:安徽农业大学, 2010. [11] 杨润军, 李青旺, 赵蕊.四氧嘧啶与链脲佐菌素诱导小鼠糖尿病模型效果的比较[J].西北农林科技大学学报, 2006, 34 (2) :17-20. [12] 张加雄, 范开华, 万丽, 等.小鼠高脂血症模型建立方法的改进[J].中国药师, 2006, 9 (8) :723-724. [13] 熊静悦, 周桢昊, 赵璐, 等.脂肪乳剂建立高脂血症小鼠的比较研究[J].中国药学刊, 2006, 24 (8) :1507-1508. [14] 李运, 段博文, 卫阳飞, 等.复方尼阿措提取物对高脂血症大鼠的调血脂作用[J].中国新药杂志, 2011, 20 (20) :2022-2025. [15] 闫志芳, 王永辉, 刘季英, 等.建立高血脂症动物模型的实验研究[J].山西中医学院学报, 2004, 5 (4) :15-16. [16] Castelli WP, Anderson K, Wilson PW, et al.Lipids and risk of coronary heart disease.The F:amingham Study[J].Annals of Epidemiology, 1992 (2) :23-28.
[17] 蔡秀成, 于力光, 邢立新, 等.松籽油对大鼠血脂及脂质过氧化的影响[J].食品科学, 1999, 20 (6) :54-57.
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