三种浸提方法从普通小球藻中提取油脂的比较研究
详细信息Study on comparison of three different extraction methods to extract oil from Chlorella vulgaris
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摘要: 分别采用直接浸出、索氏提取和超声波辅助提取三种方法从小球藻（Chlorella vulgaris C9-JN2010）藻粉中提取油脂。选择了三种方法各自的最适提取剂,研究了提取时间对提取率的影响以及提取方法对小球藻油脂肪酸组成的影响。结果表明,三种方法的提取率最高可分别达到84.06%、89.11%和88.66%,所得油脂在脂肪酸组成上无明显差别。超声波辅助提取法的提取效率最高、具有工业放大的前景。小球藻油中亚麻酸、亚油酸等多不饱和脂肪酸含量较高,可作为功能性油脂进行开发。Abstract: Leaching method, Soxhlet method and ultrasonic-assisted method were selected to obtain oil from Chlorella vulgaris C9-JN2010. Both the effect of extraction time on extraction ratio and the effect of extraction methods on fatty acids composition were studied after the best slovent of each method were selected. The results showed that the extraction ratio of leaching method, Soxhlet extraction method and ultrasonic-assisted extraction method could come up to 84.06%, 89.11% and 88.66%, respectively. And the lipid obtained by those three methods was not significantly different in fatty acids composition. As the most efficiency method, ultrasonic-assisted method was suitable for industrial amplification. The oil from Chlorella vulgaris C9-JN2010 had potential to be functional oil because of the high content of unsaturated fatty acid, such as linolenic acid and linoleic acid.
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