产细菌素屎肠球菌E6的特性分析及发酵条件优化
详细信息Characteristics and optimization of fermentative condition for bacteriocin producing Enterococcus Faecium E6
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摘要: 研究了屎肠球菌E6抑菌活性的生物学特性,并通过响应面法优化其发酵条件。结果表明,屎肠球菌E6产蛋白质类细菌素,能够抑制单增李斯特氏菌、大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌生长,在pH3.0～7.0条件下有明显抑菌活性,60～121℃热处理20min后仍具有抑菌活性。通过Box-Behnken实验设计优化屎肠球菌E6发酵条件为培养时间36.0h,培养温度31.0℃,培养基pH5.1。在此条件下,发酵上清液抑菌圈直径可达20.17mm,较优化前提高了27.2%。Abstract: The characteristics of antimicrobial activity and the fermentative condition of Enterococcus Faecium E6 were discussed.Proteinic bacteriocin produced by E6 showed inhibitory activity against Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Bacillus subtilis.It showed obviously activity under pH3.0～ 7.0 condition.It was also active when heated at 60～ 121℃ for 20min.The fermentative condition, optimized by Box-Behnken design, was cultured at pH 5.1, temperature of 31.0℃ for 36.0h.Under this condition, the diameter of inhibition zone reached up to 20.17mm, which increased by 27.2% than optimization before.
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[1] Jacob F, Lwaff A, Siminonvich A, et al.Definition de qualques terms relatifsàla lysogenie[J].Annales de Institut Pasteur, 1953, 84:222-224.
[2] Cotter PD, Hill C, Ross RP.Bacteriocins:developing innateimmunity for food[J].Nature Reviews Microbiology, 2005, 3 (10) :777-788.
[3] 田晓乐, 孟庆繁, 周杰, 等.微生物防腐剂——细菌素的研究与应用[J].食品工业科技, 2004, 25 (1) :120-123。 [4] Jiang Jie, Shi Bo, Zhu Deqiang, et al.Characterization of a novel bacteriocin produced by Lactobacillus sakei LSJ618isolated from traditional Chinese fermented radish[J].Food Control, 2012, 23 (2) :338-344.
[5] Dal Bello B, Cocolin L, Zeppa G, et al.Technological characterization of bacteriocin producing Lactococcus lactis strains employed to control Listeria monocytogenes in Cottage cheese[J].International Journal of Food Microbiology, 2012, 153 (1-2) :58-65.
[6] Cheigh Chan-Ick, Choi Hak-Jong, Park Hoon, et al.Influence of growth conditions on the production of a nisin-like bacteriocin by Lactococcus lactis subsp.lactis A164isolated from kimchi[J].Journal of Biotechnology, 2002, 95 (3) :225-235.
[7] Evrim Gunes Altuntas, Serap Cosansu, Kamuran Ayhan.Some growth parameters and antimicrobial activity of a bacteriocin-producing strain Pediococcus acidilactici13[J].International Journal of Food Microbiology, 2010, 141 (1-2) :28-31.
[8] 罗海, 唐洁, 汪静心, 等.响应曲面法优化乳杆菌产细菌素的条件研究[J].食品工业科技, 2012, 33 (13) :163-166. [9] 陈琳, 孟祥晨.响应面法优化植物乳杆菌代谢产细菌素的发酵条件[J].食品科学, 2011, 32 (3) :176-180. [10] 李玉锋, 唐洁, 车振明.工科微生物学实验[M].成都:西南交通大学出版社, 2007:28-36.
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