酶解条件对花生粕水解液的抗氧化活性影响研究

详细信息

Study on effect of enzymatic conditions on the oxidation resistance of peanut meal hydrolyzate

摘要: 以花生粕为原料,经Alcalase碱性蛋白酶水解,研究不同加酶量、底物浓度、酶解温度、酶解时间和酶解pH对花生粕水解液抗氧化性的影响。在单因素分析的基础上采用响应面分析方法对花生粕的酶解条件进行优化,以羟基自由基清除能力为考察指标,确定最佳的酶解条件为:加酶量11820U/g,底物浓度为7.52%,酶解温度为43.1℃,酶解时间为3.9h,酶解pH为8.47,羟基自由基清除能力为60.54%,在上述优化后的工艺条件下的验证实验测得羟基自由基清除能力为60.21%。
- 花生粕; /
- 酶水解; /
- 羟基自由基清除能力;
Abstract: With the peanut meal as material, Alcalase enzyme was used to hydrolyze peanut meal to prepare antioxidative hydrolyzate. The effect of enzyme dosage, substrate concentration, temperature, time and pH on oxidation resistance of peanut meal hydrolyzate was investigated. Single factor experiments and response surface test were applied to analyze the factors. The contribution rate of factors was as follow:enzyme dosage 11820U/g, substrate concentration 7.52%, temperature 43.1℃, time 3.9h and pH8.47, hydroxyl radical scavenging capacity could reach 60.54%, at this optimization condition validation experiment measured hydroxyl radical scavenging capacity was 60.21%.
- peanut meal;enzymatic hydrolysis;hydroxyl radical scavenging capacity; /
- undefined /
- undefined

[1]	刘阳, 邢福国.花生蛋白的开发和利用现状[J].食品科技, 2008, 33 (12) :173-176.
[2]	王建化, 熊柳, 孙高飞, 等.花生抗氧化活性肽制取工艺的研究[J].中国油脂, 2008, 33 (6) :15-18.
[3]	陈庆华.花生蛋白酶解特性研究[J].江苏农业科学, 2009 (3) :320-322.
[4]	吴肖, 刘通讯, 林勉.复合酶水解花生粕蛋白工艺研究[J].食品工业科技, 2005, 26 (1) :120-121.
[5]	黄文, 谢笔钧, 王益, 等.白果蛋白质的分离、纯化、理化特性及其抗氧化活性研究[J].中国农业科学, 2004, 37 (10) :1537-1543.
[6]	Shu YaoTsai, Shih Jeng Huang, Jeng Leun Mau.Antioxidant properties of hot water extracts from Agrocybe cylindracea[J].Food Chemistry, 2006, 98 (6) :670-677.
[7]	仪凯, 周瑞宝.中性蛋白酶水解花生粕的研究[J].中国油脂, 2005, 30 (7) :71-73.
[8]	王镜岩, 朱圣庚, 徐长发.生物化学:上册[M].第3版.北京:高等教育出版社, 2002:123-126.