樟芝固态发酵生产Antroquinonol及萃取Antroquinonl的研究
详细信息Research of A. camphorata solid-state fermentation production Antroquinonol and extraction Antroquinonl
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摘要: 安卓奎诺尔(Antroquinonol)具有很好的抗癌效果。为了提高樟芝固态发酵中Antroquinonol的产量及其萃取效率,为其工业化生产提供理论参考,本文考察了谷物种类、装料量、初始含水量、外加碳源、氮源含量对樟芝固态发酵产Antroquinonl的影响,并进行了多级逆流浸提方法萃取樟芝固态发酵产品中Antroquinonol的实验。结果表明:优化后的条件为:在1L三角瓶中,大米装料量为110g,初始含水量为50%,外加碳源为2.0g葡萄糖,氮源添加量为0.3g大豆粉。固态发酵中Antroquinonol产量提高了14.44%;用体积分数为95%的乙醇作萃取剂(乙醇与萃取物料比例为10∶1,v/w),以50℃水浴振荡萃取1h为浸提条件进行三级逆流浸提,浸提萃取后残渣中的Antroquinonol残留率小于1%。该法具有溶剂使用量少,萃取效率高的优点。
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关键词:
- 多级逆流萃取; /
- 樟芝; /
- 固态发酵; /
- 优化; /
- Antroquinonol;
Abstract: Antroquinonol, a metabolite produced from solid-state fermentation of Antrodia camphorata has been shown great anti-cancer effect. This study investigated the effects of a number of factors including fermented grain types, substrate capacity, initial moisture content (containing the water of seeds) , supplementary carbon source (glucose) , nitrogen content on Antroquinonol content by Antrodia cinnamomea and the multi-stage countercurrent extraction method for extracting Antroquinonol from the solid-state fermentation products of Antrodia camphorata in order to improve the production of Antroquinonol in solid-state fermentation, the extraction efficiency, decrease loss and provide theoretical basis for preparation of Antroquinonol at industry scale. The results were as follows:the best compositions of fermentation medium for Antroquinonol content were as follows:rice 110g, initial moisture content 50% (with inoculated seed liquid water) , carbon source 2.0g (glucose) , nitrogen content 0. 3g (soybean powder) . With these optimum conditions, Antroquinonol content increased by 14.44% than the initial value;After three-stage countercurrent extraction by 95% ethanol (volume ratio of ethanol and fermentation product was 10∶1, v/w) in 50°C water bath and shaking for 1h, the results showed that the residual rate of Antroquinonol in the fermentation products was less than 1%. This method showed the less solvent used and higher extraction efficiency. -
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