高效液相色谱法快速测定紫贻贝中的牛磺酸

doi:10.13386/j.issn1002-0306.2013.04.017

高效液相色谱法快速测定紫贻贝中的牛磺酸

Fast determination of taurine in Mytilus edulis Linnaeus by high-performance liquid chromatography

摘要

摘要: 目的:建立用高效液相色谱（HPLC）快速测定紫贻贝中牛磺酸含量的方法。方法:应用邻苯二甲醛（OPA）为衍生试剂进行柱前衍生,采用甲醇、乙腈和水（10:10:80,v/v/v）为流动相,色谱柱为Agilent Zorbax300SB-C18（4.6mm×250mm,5μm）,荧光检测:Ex为330nm,Em为530nm,柱温30℃,流速0.8mL/min,进样20μL分析检测牛磺酸含量。结果:牛磺酸在4.0～20.0μg/mL浓度范围内有良好线性关系,相关系数R2为0.9998,回收率范围在96.65%～101.20%,RSD=1.57%。结论:该方法的样品前处理与衍生化简便、易操作,牛磺酸出峰时间为6.752min,其含量为3673.631mg/kg,有良好的精确度和准确度,分析时间短,可快速检测紫贻贝中的牛磺酸。

Abstract: Objective:To establish the method for fast determination of taurine in Mytilus edulis Linnaeus by high performance liquid chromatography (HPLC) .Methods:The content of taurine in Mytilus edulis Linnaeus was determined by HPLC at the FLD detector excitation and emission wavelengths of 330nm and 530nm through Agilent Zorbax 300SB-C 18 (4.6mm×250mm, 5μm) column with pre-column o-pthaldialdehyde derivatization.The column temperature was 30℃, methanol-acetonitrile-water (10:10:80) was used as the mobile phase at the flow rate of 0.8mL/min.The injection volume was 20μL.Results:The taurine was in the range of 4.0～20.0μg/mL with good linear correlation, correlation coefficient R 2 =0.9998, the recoveries were 96.65% ～101.20%, RSD = 1.57%.Conclusions:The determination method was accurate with good reproducibility.The retention time of taurine was 6.752min, the taurine content was 3673.631mg/kg wet weight.The method can be used for taurine contents determination in Mytilus edulis Linnaeus.