乳酸菌蛋白水解体系及相关基因表达的研究进展
详细信息Research progress in proteolysis system of lactic acid bacteria and related gene expression
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摘要: 乳酸菌的蛋白水解体系包括胞外酶、转运系统和多种胞内酶。它们将外源蛋白质逐步水解成能被乳酸菌直接利用的游离氨基酸,弥补了因乳酸菌自身不能直接利用外源无机氮和蛋白质的缺陷,对于乳酸菌的正常生长具有非常重要的意义。目前,国内外正在研究利用现代分子技术,从基因表达层面检测不同菌株之间或菌株经过不同处理前后关键蛋白水解酶的表达水平差异,筛选出蛋白酶高表达量的菌株,从而得到蛋白水解能力强的优质乳酸菌发酵剂菌株。本文将对乳酸菌的蛋白水解体系及近几年乳酸菌蛋白水解体系中相关基因表达情况的研究进展进行综述,以期为乳酸菌蛋白质代谢的进一步研究提供参考。Abstract: The proteolytic system of lactic acid bacteria which hydrolyzes exogenous protein into free amino acid were consists of extracellular peptidases, the peptide transport systems and a variety of intracellular peptidases. That makes up of the deficiency that lactic acid bacteria can not directly use inorganic nitrogen and protein. Therefore the proteolytic system has very important significance for the normal growth of lactic acid bacteria. Currently some scientists use the modern molecular techniques to study the gene expression levels of the key proteolytic enzymes of the different strains or the strains after different treatments in order to screen out the strains in which the genes of proteinases has high expression level and obtain the high-quality starter lactic acid bacteria strains.The proteolytic system of lactic acid bacteria and the advances in the expression level of genes related to the proteolytic system of lactic acid bacteria were reviewed in this paper with the hope of providing reference materials for the further study on protein metabolism of lactic acid bacteria.
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