混菌降解稻草纤维素产葡萄糖的条件研究
详细信息Study on the condition of straw cellulose degradation to produce glucose by mixed culture
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摘要: 利用从环境中筛选出的一株木霉与一株曲霉,在以稻草粉为主要碳源的液体培养基中混菌发酵,所得的粗酶液催化降解经预处理的稻草粉得到葡萄糖液。在单因素实验基础上进行正交实验,正交实验表明,粗酶液用量对稻草粉降解为葡萄糖影响最大,其次为温度,然后是pH,稻草粉浓度对降解影响最小。稻草粉最优降解条件为:粗酶液用量为降解液总体积的3%,降解液初始稻草粉浓度60gL,pH4.8,降解温度50℃,此条件下降解率可达80%。Abstract: In this research, a strain of trichoderma and aspergillus filtered out from the environment was used to ferment in powder-liquid medium where the main carbon source was rice straw.Along with the fermentation, the pretreated rice straw powder was catalytic degraded into glucose by enzyme.There was orthogonal test based on single factor experiment.The orthogonal experiment showed that the crude enzyme liquid dosage caused the biggest influence on the degradation of straw powder, second was temperature and followed by pH value, and the straw powder concentration caused the minimum effects on degradation.The optimal conditions of degradation of rice straw powder was:the percentage of the volume of crude enzyme liquid in total degradation liquid was 3%, initial straw powder concentration was 60g/L, the pH value was 4.8, the degradation temperature was 50℃.Under the optimal conditions, the degradation rate could be able to reach 80%.
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Keywords:
- mixed culture;degradation;rice straw;cellulose; /
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