离子色谱法测定奶糖中的蔗糖、葡萄糖和乳糖
详细信息Determination of sucrose, glucose and lactose in toffee by ion chromatography
-
摘要: 建立离子色谱法分离检测奶糖中蔗糖、葡萄糖和乳糖的分析方法。采用METROSEPCARB1(150mm×4.0mm)阴离子交换分离柱和脉冲安培检测器,对淋洗分离条件进行优化。确定柱温为32℃、淋洗液为37.0mmol/L NaOH溶液,淋洗液流速为1.0mL/min,总分析时间20min。在1.0～60.0mg/L范围内蔗糖、葡萄糖和乳糖分别呈良好线性,相关系数分别为0.9996、0.9998和0.9996,检出限分别为0.091、0.014和0.083mg/L,RSD为1.42%～5.17%,回收率为91.27%～101.31%。测定结果为大白兔奶糖中含葡萄糖1.56%、蔗糖21.40%、果糖3.75%;旺仔牛奶糖中含葡萄糖1.17%、蔗糖30.29%、果糖3.30%;金丝猴奶糖中含葡萄糖1.08%、蔗糖20.86%、果糖3.31%。用该方法检测了奶糖中的三种糖,效果良好,同时具有操作简单,结果准确,快速有效等优点,可为奶糖的检测提供一个简单有效的方法。Abstract: An ion chromatographic method had been developed for the determination of sucrose, glucose and lactose in toffee.The optimal chromatographic conditions were investigated for the separation on METROSEP CARB 1 (150mm×4.0mm) anion exchange column.Pulsed amperometric detector was used for detection.Column temperature was 32℃.The separation was performed using 37.0mmol/L NaOH at a flow rate of 1.0mL/min.The total analysis time was 20min.The linear ranges of sucrose, glucose and lactose were 1.0～60.0mg/L, respectively.Their correlation coefficients were 0.9996, 0.9998 and 0.9996.The detection limits were 0.091, 0.014 and 0.083mg/L, correspondingly.The relative standard deviation was between 1.42% and 5.17%.The recoveries were between 91.27% and 101.31%.The results of the samples showed that glucose content of white rabbit toffee was 1.56%, sucrose content was 21.40%, lactose content was 3.75%.Glucose content of Wangzai toffee was 1.17%, sucrose content was 30.29%, lactose content was 3.30%.Glucose content of golden monkey toffee was 1.08%, sucrose content was 20.86%, lactose content was 3.31%.In the application of the method to the analysis of toffee satisfactory results were obtained.At the same time, this method was characteristic of simple operation, the accurate results and shorter analysis time.It could provide a simple and efficient method for the determination of toffee.
-
[1] Cristiana Campa, Edi Baiutti, Anna Flamigni.Capillary electrophoresis of sugar acids[J].Methods in Molecular Biology, 2008, 384 (1) :307-355.
[2] 赵燕, 刘莉, 杨兴斌, 等.高效毛细管电泳法测定唐古特大黄多糖中单糖的组成[J].医药导报, 2005, 24 (11) :981-983. [3] 张威, 何洪波, 张明, 等.糖腈乙酰酯衍生气相色谱法测定土壤水解性单糖[J].土壤通报, 2008, 39 (4) :913-916. [4] 周欣, 王庆彪, 刘锡钧, 等.气相色谱法检测葡萄糖、麦芽糖、果糖和蔗糖[J].海峡药学, 2001, 13 (4) :48-49. [5] 孟娟.蒽酮-硫酸比色法测定甘草残渣中多糖含量[J].天津化工, 2011, 25 (5) :45-46. [6] 郭玉华, 杨伟华, 郁有祝, 等.蒽酮比色法测定棉花成熟纤维中水溶性总糖含量[J].2011, 38 (12) :23-26. [7] 孟伊娜, 张瑞廷, 史强, 等.高效液相色谱法测定甜高粱茎秆中3种糖含量[J].中国农学通报, 2010, 26 (8) :90-95. [8] Salim Ouchemoukh, Paul Schweitzer, Mostapha Bachir Bey, et al.HPLC sugar profiles of Algerian honeys[J].Food Chemistry, 2010, 121 (2) :561-568.
[9] 余娜, 周光明.离子色谱法测定苦瓜茶中的半乳糖、葡萄糖、甘露糖和果糖[J].食品科技, 2011, 36 (8) :266-269. [10] ALBERTSON P L, ROF C P L.Application of high performance anion exchange-pulsed amperometric detection to the activity of key sucrose metabolizing enzymes in sugarcane[J].Journal of Chromatography B, 2007, 845 (1) :151-156.
[11] MOU Shi-fen, YU Hong, YA Qi.Analysis of sugars by high performance anion-exchange chromatography with pulsed amperometric detection[J].2009, 27 (5) :667-674.
[12] Ji-Seon Jeong, Ha-Jeong Kwon, Yong-Moon Lee, et al.Determination of sugar phosphates by high-performance anion-exchange chromatography coupled with pulsed amperometric detection[J].2007, 1164 (1-2) :167-173.
[13] 许丽, 周光明, 余娜, 等.超声提取-离子色谱法测定蜂蜜柚子茶中的可溶性糖[J].西南大学学报, 2011, 33 (11) :90-94. [14] 黄晓书, 徐鹏, 李卫华, 等.高效阴离子交换色谱-脉冲安培检测法分析蜜枣中的糖类化合物[C], 第十二届全国离子色谱学术报告会论文集, 2008, 11 (5-8) :440-443. [15] Cataldi TRI, Angelotti M, Bianco G.Determination of mono and disaccharides in milk and milk products by high-performance anion-exchange chromatography with pulsed amperometric detection[J].2003, 485 (1) :43-45.
[16] 周帅, 薛俊杰, 刘艳芳, 等.高效阴离子色谱-脉冲安培检测法分析食用菌中海藻糖、甘露醇和阿糖醇[J].食用菌学报, 2011, 18 (1) :49-52.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
