肉桂水提物对大鼠肠道梭菌属IV簇细菌和拟杆菌的影响
详细信息Effect of aqueous Cinnamomum cassia extracts on gastrointestinal Clostridium cluster IV and Bacteroides in rats
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摘要: 目的:肉桂水提物对大鼠结肠和直肠梭菌属IV簇和拟杆菌的影响。方法:给SD大鼠灌胃一个月后分别取结肠和直肠内容物,针对肠道梭菌属IV簇和拟杆菌的16SrRNA基因应用末端限制性片段多态性分析技术对这两类菌的多样性及分布进行分析。结果:研究发现肉桂水提物能降低梭菌属IV簇细菌在结肠和直肠中的数量,增加拟杆菌属细菌在结肠和直肠中的数量;该水提物对结肠和直肠的结构均产生影响,但影响并不相同,其中对直肠的影响更大。结论:肉桂水提物对大鼠肠道菌群的这种影响可能与肉桂治疗肥胖和糖尿病有功效有关。Abstract: Objective:To explore the impact of aqueous Cinnamomum cassia (C.cassia) extracts on gut microbiota.Methods:The colon and rectal contents were sampled after rats had been orally administrated the aqueous extracts for one month.Clostridium cluster IV and Bacteroides in the samples were analyzed by the terminal restriction fragment length polymorphism (tRFLP) method based on the bacterial 16S rRNA gene and nucleotide sequencing.Result:The aqueous C.cassia extracts could down-regulate the amount of microbiota of Clostridium cluster IV in rat gut, along with the increase of microbiota of Bacteroides.Various effects were exerted by the extracts on microbiota in colon and rectum, and the impact presented heavier in rectum.Conclusion:These variations of rat gut microbiota induced by the aqueous C.cassia extracts may reflect the functions of anti-obese and anti-dyspepsia of C.cassia.
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[1] Lee R, Balick MJ.Sweet wood-cinnamon and its importance as a spice and medicine[J].Explore (NY) , 2005, 1 (1) :61-64.
[2] Lee JS, Jeon SM, Park EM, et al.Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense systems in high cholesterol-fed rats[J].J Med Food, 2003, 6 (3) :183-191.
[3] Marsh TL.Terminal restriction fragment length polymorphism (T-RFLP) :an emerging method for characterizing diversity among homologous populations of amplification products[J].Curr Opin Microbiol, 1999, 2 (3) :323-327.
[4] Matan N, Rimkeeree H, Mawson AJ, et al.Antimicrobial activity of cinnamon and clove oils under modified atmosphere conditions[J].Int J Food Microbiol, 2006, 107 (2) :180-185.
[5] Youn HS, Lee JK, Choi YJ, et al.Cinnamaldehyde suppresses toll-like receptor4activation mediated through the inhibition of receptor oligomerization[J].Biochem Pharmacol, 2008, 75 (2) :494-502.
[6] Couturier K, Batandier C, Awada M, et al.Cinnamon improves insulin sensitivity and alters the body composition in an animal model of the metabolic syndrome[J].Arch Biochem Biophys, 2010, 501 (1) :158-161.
[7] Kwon HK, Hwang JS, So JS, et al.Cinnamon extract induces tumor cell death through inhibition of NFκB and AP1[J].BMC Cancer, 2010 (10) :392-401.
[8] Wang R, Wang RJ, Yang B.Extraction of essential oils from five cinnamon leaves and identification of their volatile compound compositions[J].Innov Food Sci Emerg, 2009, 10 (2) :289-292.
[9] Laparra JM, Sanz Y.Interactions of gut microbiota with functional food components and nutraceuticals[J].Pharmacol Res, 2010, 61 (3) , 219-225.
[10] Woodmansey EJ.Intestinal bacteria and ageing[J].J Appl Microbiol, 2007, 102:1178-1186.
[11] Bernhard AE, Field KG.A PCR assay to discriminate humanand ruminant feces on the basis of host differences in BacteroidesPrevotella genes encoding16S rRNA[J].Appl Environ Microbiol, 2000, 66 (10) :4571-4574.
[12] Matsuki T, Watanabe K, Fujimoto J, et al.Use of16S rRNA gene-targeted group-speci.c primers for real-time PCR analysis of predominant bacteria in human feces[J].Appl Environ Microbiol, 2004, 70:7220-7228.
[13] Dunbar JM, Ticknor LO, Kuske CR.Phylogenetic specificity and reproducibility and new method for analysis of terminal restriction fragment profiles of16S rRNA genes from bacterial communities[J].Appl Environ Microbiol, 2001, 67:190-197.
[14] Mills DK, Fitzgeralda K, Litchfielda CD, et al.A comparison of DNA profiling techniques for monitoring nutrient impact on microbial community composition during bioremediation of petroleumcontaminated soils[J].J Microbiol Methods, 2003 (4) :57-74.
[15] Falk MW, Song KG, Matiasek MG, et al.Microbial community dynamics in replicate membrane bioreactors-natural reproducible fluctuations[J].Water Res, 2009, 43:842-852.
[16] Khafipour E, Li S, Plaizier JC, et al.Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J].Appl Environ Microbiolm, 2009, 75:71157124.
[17] Clarke KR, Warwick RM.Change in Marine Communities:An Approach to Statistical Analysis and Interpretation[M].2nd Ed.PRIMER-E, Plymouth Marine Laboratory, Plymouth, UK, 2001:172.
[18] Rees GN, Baldwin DS, Watson GO, et al.Ordination and significance testing of microbial community composition derived from terminal restriction fragment length polymorphisms:application of multivariate statistics[J].Antonie van Leeuwenhoek, 2004, 86:339-347.
[19] Lee HS, Ahn YJ.Growth-Inhibiting Effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria[J].J Agri Food Chem, 1998, 46 (1) :8-12.
[20] Spencer JPE.Metabolism of tea flavonoids in the gastrointestinal tract[J].J Nutr (supplement) , 2003:3255s-3261s.
[21] Lee HC, Jenner AM, Low CS.Effect of tea phenolics and their aromatic fecal bacterial metabolites on intestinal microbiota[J].Res Microbiol, 2006, 157 (9) :876-884.
[22] Turnbaugh PJ, Hamady M, Yatsunenko T, et al.A core gut microbiome in obese and lean twins[J].Nature, 2009, 457:480485.
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