大孔吸附树脂分离纯化桃花多酚的研究
Separation and purification of peach blossom polyphenols with macroporous resins
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摘要: 目的:研究大孔吸附树脂分离纯化桃花多酚的最佳工艺条件。方法:比较了6种大孔吸附树脂对桃花多酚的静态吸附与解吸性能,筛选出最佳树脂并对其进行开云（中国）吸附、解吸实验,确定最佳纯化桃花多酚工艺条件。结果:D-101树脂具有较好的吸附解吸效果。最佳工艺为:上样浓度约为0.744mg/mL,上样流速1BV/h,上样体积4.5BV,先用去离子水洗至洗脱液无色,再用80%乙醇以1BV/h的流速进行洗脱,洗脱液用量约3BV。该条件下桃花多酚的质量分数可从6.17%提高到29.30%±3.04%,桃花多酚的总收率达79.01%±3.39%。结论:该方法简单可行,效果较好,可满足工业生产要求。Abstract: Objective:To study the optimal separation and purification technological parameters of peach blossom polyphenols with macroporous resins.Methods:6 types of macroporous resins were compared through static experiments.D-101 resin was screened out for higher adsorption and desorption performances, and then its dynamic adsorption-desorption properties were investigated to get the optimum parameters.Results:D-101 resin was suitable for purifying peach blossom polyphenols.The optimum technology parameters were as follows:4.5BV sample added on the column, and the sample concentration was about 0.744mg/mL at the flow rate of 1BV/h;then washed by deionized water to the outflow nearly colorless, the desorption was eluted with 80% alcohol at flow rate of 1BV/h, the eluant volume was about 3BV.The peach blossom polyphenols purity increased from 6.17% to 29.30%±3.04% and the total polyphenols yield reacheed 79.01%±3.39%, under the optimum conditions.Conclusion:The method was simple and feasible with good effect of separation, which could meet the industrial requirements.
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