响应面法优化Coriolus versicolor ZZH-2产漆酶培养基
详细信息Medium optimization of Coriolus versicolor ZZH-2 for laccase production by response surface methodology
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摘要: 采用响应面法对Coriolus versicolor ZZH-2产漆酶培养基进行优化,首先用Plackett-Burman（PB）法筛选出3个影响较大的重要因素,分别为酵母膏、羧甲基纤维素钠和L-天冬氨酸。在此基础上,用最陡爬坡实验逼近最大响应区域,并利用中心组合实验以及响应面分析确定了主要影响因素的最佳条件,即酵母膏13.82g/L、羧甲基纤维素钠13.29g/L和L-天冬氨酸1.44g/L,在优化培养基后漆酶酶活达到9693U/mL,比优化前提高了1.7倍,其实验值与理论值基本相符。Abstract: In this report, response surface methodology was applied to optimize the fermentation conditions Coriolus versicolor ZZH-2 for Laccase Production. Firstly, Plackett-Burman design was used to select the main factors affecting laccase yield of Coriolus versicolor ZZH-2. The results showed that three factors played important roles in the fermentation conditions, including the concentration of yeast extract, sodium carboxymethyl cellulose (CMC) and L-aspartic acid. The pass of steepest ascent was undertaken to approach the optimal region of the three significant to factors. Then the central composite design and response surface analysis were applied to investigate the mutual interaction between the variables and identify optimal values that bring maximum laccase activity. The activity of laccase increased nearly 1.7 times to 9693U/mL where concentrations of yeast extract, sodium carboxymethyl cellulose (CMC) and L-aspartic acid were at 13.82, 13.29 and 1.44g/L. The experimental values agreed with the predicted values.
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