离子交换法提取酶促转化液中的L-茶氨酸
详细信息Purification of L-theanine from enzymatic catalyzed reaction solution by ion-exchange resins
-
摘要: 用717阴离子交换树脂提取酶促转化液中的L-茶氨酸,用静态和动态的方法考察了不同操作条件对固定床离子交换效果的影响。静态吸附实验表明,717阴离子交换树脂对酶促转化液中L-茶氨酸的最佳吸附条件为pH8.5,平衡吸附时间16min,吸附量为0.083mmol/g。L-茶氨酸转化液上样的最佳流速为0.5BV/h。按照以上条件,转化液稀释3倍上样,经0.5mol/L盐酸洗脱,旋转蒸发仪浓缩,醇沉结晶后,可得L-茶氨酸纯品。Abstract: L-theanine was isolation and purification from enzymatic catalyzed reaction solution by 717 anion-exchange resins.Through static and dynamic experiments, different operating conditions in the fixed bed to separate L-theanine were investigated.The results showed that the optimal conditions were pH8.5, adsorption balance time was 16min and exchange capacity was 0.083mmol/g in static experiment.The optimal flow rate was 0.5BV/h in dynamic experiment.Under these conditions, the reaction solution was diluted with water, added into the ion exchange column and eluted by 0.5mol/L hydrochloric acids.White crystal of L-theanine was obtained after concentrated, crystallized and dryied.
-
[1] Eschenauer G, Sweet BV.Pharmacology and therapeutic uses of theanine[J].American Journal of HealthSystem Pharmacy, 2006, 63 (1) :26-30.
[2] Graham HN.Green tea composition and polyphenol chemistry[J].Preventive Medicine, 1992, 21 (3) :334-350.
[3] Juneja LR, Chu DC, Okubo T, et al.Ltheaninea unique amino acid of green tea and its relaxation effect in humans[J].Trends in Food Science&Technology, 1999, 10 (6-7) :199-204.
[4] Matsuura T, Sakane I, Kakuda T, et al.Effects of plant growth regulators and carbon sources on theanine formation in callus cultures of tea (Camellia sinensis) [J].Bioscience, Biotechnology, and Biochemistry, 1994, 58 (6) :1519-1521.
[5] Orihara Y, Furuya T.Production of theanine and otherγ-glutamyl derivatives by Camellia sinensis cultured cells[J].Plant Cell Reports, 1990, 9:65-68.
[6] Gu Haining, Jiang Yongxiang, Wang Jiong.A practical synthesis of ethyl L glutamine (L theanine) [J].Organic Preparations and Procedures International, 2004, 36 (2) :182-185.
[7] Lichtenstein N.Preparation ofγ-alkylamides of glutamic acid[J].Journal of the American Chemical Society, 1942, 64 (5) :1021-1022.
[8] He Yanshou, Dufour JP, Meurens M.高纯度茶氨酸的合成与特性[J].茶叶科学, 2003, 23 (2) :99-104. [9] Suzuki H, Izuka S, Miyakawa N, et al.Enzymatic production of theanine, an“umami”component of tea, from glutamine and ethylamine with bacterialγglutamyltranspeptidase[J].Enzyme and Microbial Technology, 2002, 31 (6) :884-889.
[10] Tachiki T, Yamada T, Mizuno K, et al.γGlutamyl transfer reactions by glutaminase from Pseudomonas nitroreducens IFO12694and their application for the syntheses of theanine and g glutamylmethylamide[J].Bioscience, Biotechnology, and Biochemistry, 1998, 62 (7) :1279-1283.
[11] Yamamoto S, Wakayama M, Tachiki T.Theanine production by coupled fermentation with energy transfer employing Pseudomonas taetrolens Y30glutamine synthetase and baker’s yeast cells[J].Bioscience, Biotechnology, and Biochemistry, 2005, 69 (4) :784-789.
[12] Hung CP, Lo HF, Hsu WH, et al.Immobilization of Escherichia coli novablueγglutamyltranspeptidase in Ca-alginatek carrageenan beads[J].Applied Biochemistry and Biotechnology, 2008, 150 (2) :157-170.
[13] 张飞, 熊吉滨, 刘均忠, 等.L-谷氨酰肼为底物酶法制备茶氨酸[J].精细化工, 2010, 27 (2) :130-132. [14] Gu Haining, Jiang Yongxiang, Wang Jiong.A practical synthesis of ethyl L glutamine (L theanine) [J].Organic Preparations and Procedures International, 2004, 36 (2) :182-185.
计量
- 文章访问数:
- HTML全文浏览量:
- PDF下载量:
下载:
